| Course Unit Code | Course Unit Title | Type of Course Unit | Year of Study | Semester | Number of ECTS Credits | | GIM-23-147 | PURIFICATION METHODS OF PROTEINS | Elective | 1 | 2 | 6 |
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| Level of Course Unit |
| Second Cycle |
| Objectives of the Course |
| Having information about purification of proteins |
| Name of Lecturer(s) |
| Dr. Öğr. Üyesi Uğur GÜLLER |
| Learning Outcomes |
| 1 | At the end of this course the student;
will learn the purification of protein from any material. |
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| Mode of Delivery |
| Daytime Class |
| Prerequisites and co-requisities |
| none |
| Recommended Optional Programme Components |
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| Course Contents |
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| Weekly Detailed Course Contents |
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| 1 | The general structure of amino acids and proteins | | | | 2 | Conformation and configuration concepts in proteins | | | | 3 | Denaturation of proteins | | | | 4 | Cell disruption methods for protein extraction | | | | 5 | Isolation of protein from the cell components | | | | 6 | The techniques used in the purification | | | | 7 | Protein separation methods (according to molecular size difference) | | | | 8 | Protein separation methods (according to the resolution difference) | | | | 9 | Protein separation methods (depending on the electrical charge differences) | | | | 10 | Separation of proteins by biological affinity | | | | 11 | Purification and fractionation of proteins | | | | 12 | Dialysis of the protein solutions | | | | 13 | Identification of proteins by electrophoretic method | | | | 14 | Qualitative and quantitative analysis of proteins | | |
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| Recommended or Required Reading |
| Keha, E.E., Küfrevioğlu, Ö.İ., Biyokimya, Aktif Yayınevi, İstanbul. |
| Planned Learning Activities and Teaching Methods |
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| Assessment Methods and Criteria | |
| Midterm Examination | 1 | 100 | | SUM | 100 | |
| Final Examination | 1 | 100 | | SUM | 100 | | Term (or Year) Learning Activities | 50 | | End Of Term (or Year) Learning Activities | 50 | | SUM | 100 |
| | Language of Instruction | | Turkish | | Work Placement(s) | |
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| Workload Calculation |
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| Midterm Examination | 1 | 1 | 1 |
| Final Examination | 1 | 2 | 2 |
| Attending Lectures | 14 | 3 | 42 |
| Laboratory | 1 | 12 | 12 |
| Problem Solving | 1 | 12 | 12 |
| Discussion | 1 | 6 | 6 |
| Experiment | 1 | 12 | 12 |
| Brain Storming | 1 | 12 | 12 |
| Report Preparation | 1 | 12 | 12 |
| Report Presentation | 1 | 3 | 3 |
| Project Preparation | 1 | 12 | 12 |
| Project Presentation | 1 | 12 | 12 |
| Seminar | 1 | 6 | 6 |
| Criticising Paper | 1 | 12 | 12 |
| Report | 1 | 12 | 12 |
| Homework | 1 | 12 | 12 |
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| Contribution of Learning Outcomes to Programme Outcomes |
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| * Contribution Level : 1 Very low 2 Low 3 Medium 4 High 5 Very High |
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Iğdır University, Iğdır / TURKEY • Tel (pbx): +90 476
226 13 14 • e-mail: info@igdir.edu.tr
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