| Course Unit Code | Course Unit Title | Type of Course Unit | Year of Study | Semester | Number of ECTS Credits | | GIM-23-100 | ANTIOXIDANT ENZYMES AND ACTIVITY ASSAY METHODS | Elective | 1 | 2 | 6 |
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| Level of Course Unit |
| Second Cycle |
| Objectives of the Course |
| The role and importance of enzymes in the antioxidant defense system in metabolism in oxidative stress situations and explaining the activity assay methods. |
| Name of Lecturer(s) |
| Dr.Öğr.Üyesi Elif Duygu KAYA |
| Learning Outcomes |
| 1 | At the end of this course, students will have information about antioxidant enzymes | | 2 | and will be able to determine the activity of antioxidant enzymes in plant or biological tissues alone. |
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| Mode of Delivery |
| Daytime Class |
| Prerequisites and co-requisities |
| none |
| Recommended Optional Programme Components |
| At the end of this course, students will have information about antioxidant enzymes and will be able to determine the activity of antioxidant enzymes in plant or biological tissues alone. |
| Course Contents |
| Explain the enzymes in the antioxidant defense system
To examine the change of antioxidant enzymes under oxidative stress conditions.
To examine the methods of determining the activities of antioxidant enzymes in plant materials and tissues. |
| Weekly Detailed Course Contents |
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| 1 | Reactive oxygen species, antioxidant sources | none | none | | 2 | Oxidative stress, enzymes in the antioxidant defense system | none | none | | 3 | Preparation of enzyme extracts, basic principles of activity measurement | none | none | | 4 | Protein analysis methods and determination of specific activity | none | none | | 5 | The peroxidase ezymes family and their role in the antioxidant defense system | yok | yok | | 6 | Activity determination methods of peroxidases | none | none | | 7 | Catalase enzyme and its roles in antioxidant defense system | none | none | | 8 | Catalase enzyme activity determination methods | none | none | | 9 | Superoxide dismutase enzyme and its roles in antioxidant defense | none | none | | 10 | Superoxide dismutase enzyme activity determination methods | none | none | | 11 | Phenol oxidase enzymes and their role in antioxidant defense | none | none | | 12 | Activity determination methods of phenol oxidases | none | none | | 13 | Glutathione reductase enzyme and its role in antioxidant defense system | none | none | | 14 | Activity determination methods of glutathione reductase enzyme | none | none |
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| Recommended or Required Reading |
| Barry Halliwell and John M. C. Gutteridge, Free Radicals in Biology and Medicine, Oxford Science Publications, 2001
Academic publications |
| Planned Learning Activities and Teaching Methods |
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| Assessment Methods and Criteria | |
| Midterm Examination | 1 | 100 | | SUM | 100 | |
| Final Examination | 1 | 100 | | SUM | 100 | | Term (or Year) Learning Activities | 50 | | End Of Term (or Year) Learning Activities | 50 | | SUM | 100 |
| | Language of Instruction | | | Work Placement(s) | | none |
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| Workload Calculation |
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| Midterm Examination | 1 | 1 | 1 |
| Final Examination | 1 | 2 | 2 |
| Attending Lectures | 14 | 3 | 42 |
| Brain Storming | 14 | 3 | 42 |
| Self Study | 14 | 3 | 42 |
| Individual Study for Mid term Examination | 1 | 30 | 30 |
| Individual Study for Final Examination | 1 | 30 | 30 |
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| Contribution of Learning Outcomes to Programme Outcomes |
| LO1 | 2 | 2 | 3 | 3 | 1 | 3 | 3 | 4 | 3 | 4 | 2 | 4 | 3 | 4 | 3 | 3 | | LO2 | 3 | 3 | 3 | 3 | 1 | 2 | 2 | 2 | 4 | 3 | 3 | 4 | 3 | 3 | 3 | 2 |
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| * Contribution Level : 1 Very low 2 Low 3 Medium 4 High 5 Very High |
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Iğdır University, Iğdır / TURKEY • Tel (pbx): +90 476
226 13 14 • e-mail: info@igdir.edu.tr
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