| Course Unit Code | Course Unit Title | Type of Course Unit | Year of Study | Semester | Number of ECTS Credits | | TAB-23-109 | PLANT TISSUE CULTURE AND APPLICATIONS | Elective | 1 | 1 | 6 |
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| Level of Course Unit |
| Second Cycle |
| Objectives of the Course |
| The purpose of this class, to educate students about different tissue culture methods in the plant breeding area. |
| Name of Lecturer(s) |
| Yrd. Doç. Dr. Ahmet Metin KUMLAY |
| Learning Outcomes |
| 1 | explain the basic laboratory techniques used in plant tissue culture. | | 2 | learn organization of the plant tissue culture laboratory. | | 3 | apply the application areas of plant tissue cultures in plant breeding and types of plant tissue culture. | | 4 | compare which technique is in conformity with the purpose and plant tissue. |
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| Mode of Delivery |
| Daytime Class |
| Prerequisites and co-requisities |
| None |
| Recommended Optional Programme Components |
| None |
| Course Contents |
| Basic laboratory techniques used in plant tissue culture and organization of the plant tissue culture laboratory, the sterilization techniques, the importance of nutrient media and culture conditions and practice on solution preparation, application areas of plant tissue cultures in plant breeding, types of plant tissue culture and application on micropropagation, commercial applications of plant tissue culture and to produce disease-free plants, practical applications of protoplast culture and somatic hybridization, germplasm conservation techniques. |
| Weekly Detailed Course Contents |
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| 1 | Basic laboratory techniques in Plant Tissue Culture | Instruction with slides | | | 2 | Preparation of media and stock solutions and their storage | Instruction with slides | | | 3 | Micropropagation techniques | Instruction with slides-Practice | | | 4 | Micropropagation techniques | Instruction with slides-Practice | | | 5 | Disease-free plant propagation | Instruction with slides-Practice | | | 6 | Disease-free plant propagation | Instruction with slides-Practice | | | 7 | Protoplast culture and somatic hybridization | Instruction with slides-Practice | | | 8 | Mid-Term Exam | Instruction with slides | | | 9 | Organogenesis | Instruction with slides-Practice | | | 10 | Somatic embriyogenesis and somaclonal variation | Instruction with slides | | | 11 | Root, shoot and callus cultures | Instruction with slides-Practice | | | 12 | Embryo Culture | Instruction with slides-Practice | | | 13 | Haploid plant production | Instruction with slides | | | 14 | Production of secondary metabolites via plant tissue culture | Instruction with slides | | | 15 | Germplasm Conservation | Instruction with slides | | | 16 | Final Exam | | |
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| Recommended or Required Reading |
| Bitki Biyoteknolojisi-Doku Kültürü ve Uygulamaları. Editörler: Mehmet Babaoğlu, Ekrem Gürel, Sebahattin Özcan. Selçuk Üniversitesi Vakfı Yayınları, Konya, 2001.
Plant Tissue Culture Engineering. S. Dutta Gupta and Yasuomi Ibaraki. (Series Editors: Marcel Hofman-Josef Anne) Springer Press, the Netherlands, 2008.
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| Planned Learning Activities and Teaching Methods |
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| Assessment Methods and Criteria | | | Language of Instruction | | Turkish | | Work Placement(s) | | None |
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| Workload Calculation |
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| Midterm Examination | 1 | 5 | 5 |
| Final Examination | 1 | 5 | 5 |
| Attending Lectures | 14 | 2 | 28 |
| Practice | 7 | 2 | 14 |
| Laboratory | 7 | 2 | 14 |
| Writing Paper | 1 | 30 | 30 |
| Criticising Paper | 1 | 15 | 15 |
| Individual Study for Mid term Examination | 1 | 30 | 30 |
| Individual Study for Final Examination | 1 | 40 | 40 |
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| Contribution of Learning Outcomes to Programme Outcomes |
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| * Contribution Level : 1 Very low 2 Low 3 Medium 4 High 5 Very High |
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Iğdır University, Iğdır / TURKEY • Tel (pbx): +90 476
226 13 14 • e-mail: info@igdir.edu.tr
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