| Course Unit Code | Course Unit Title | Type of Course Unit | Year of Study | Semester | Number of ECTS Credits | | TAB-23-112 | INSTRUMENTAL ANALYSIS TECHNIQUES USED FOR DETERMINATION OF PLANT COMPONENTS | Elective | 1 | 2 | 6 |
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| Level of Course Unit |
| Second Cycle |
| Objectives of the Course |
| Intrumental analysis techniques have been used for determination of plant constituents, recently. These tecniques are fundamental in biology, agriculture and food technology. In this lecture, student will learn theoritical basis of intrumental tools and will be able to use these equipments. Plant aromatic contituents, pesticide residues and other components will easily be determined at the result of the class. |
| Name of Lecturer(s) |
| Yrd. Doç. Dr. Ahmet Metin Kumlay |
| Learning Outcomes |
| 1 | Theoretical knowledge about plant components and biochemistry | | 2 | Theoretical and practical information about refractometry, polarimetry spectrophotometry (UV/VIS) | | 3 | Detailed information about chromatographic techniques and their usage on plants | | 4 | Theoretical and practical information about HPLC, GC, MS, GC-MS, LC-MS and their usage on plants | | 5 | Application of Electrophoretic Techniques on Plants | | 6 | Interpretation of results |
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| Mode of Delivery |
| Daytime Class |
| Prerequisites and co-requisities |
| None |
| Recommended Optional Programme Components |
| None |
| Course Contents |
| 1.To teach the basic principals and theory of instrumental techniques,
2.To explain theory and application of Refractometry and Polarimetry,
3.To train theory and application of Spectrophotometry (UV/VIS),
4.To educate theory and application of Chromatography,
5.To practice theory and application of Electrophoretic techniques. |
| Weekly Detailed Course Contents |
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| 1 | Basic Principals of Instrumental Analysis Techniques | | | | 2 | Refractometry and Polarimetry | | | | 3 | Spectrophotometry (UV/VIS) | | | | 4 | General Principles of High Performance Liquid Chromatography (HPLC) | | | | 5 | Detailed Information about HPLC instrument | | | | 6 | HPLC Applications on Plants (Carbohydrates, Lipids, Vitamines) | | | | 7 | HPLC Applications on Plants (Mycotoxines, Pesticide Residues, Amino Acids) | | | | 8 | Mid-Term Exam | | | | 9 | Basic Principles af Gas Chromatography (GC) and Mass Spectrophotometry (MS) | | | | 10 | GC and MS applications on plants | | | | 11 | GC-MS and LC-MS applications on plants | | | | 12 | GC-MS and LC-MS applications on plants | | | | 13 | Basic Principals of Electrophoretic Techniques | | | | 14 | Application of Electrophoretic Techniques on Plants | | | | 15 | Application of Protein and DNA Electrophoresis Techniques on Plants | | | | 16 | Final Exam | | |
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| Recommended or Required Reading |
| 1.Enstrümental Gıda Analizleri - I – Yüksek Basınç Sıvı Kromatografisi. Prof. Dr. Yaşar Hışıl. Ege Üniversitesi Mühendislik Fakültesi Ders Kitapları Yayın No:31, İzmir, 2004.
2.Enstrümental Gıda Analizleri - II – Gaz, İnce-Tabaka, Kağıt Kromatografileri ve Elektroforez. Prof. Dr. Yaşar Hışıl. Ege Üniversitesi Mühendislik Fakültesi Ders Kitapları Yayın No:30, İzmir, 2004.
3.Enstrümental Gıda Analizleri. Prof. Dr. Hasan Yetim, Atatürk Üniversitesi Ziraat fakültesi Ders Yayınları No: 224, Erzurum, 2002 |
| Planned Learning Activities and Teaching Methods |
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| Assessment Methods and Criteria | | | Language of Instruction | | Turkish | | Work Placement(s) | | None |
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| Workload Calculation |
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| Midterm Examination | 1 | 5 | 5 |
| Final Examination | 1 | 5 | 5 |
| Attending Lectures | 14 | 2 | 28 |
| Laboratory | 14 | 2 | 28 |
| Writing Paper | 1 | 30 | 30 |
| Individual Study for Mid term Examination | 1 | 30 | 30 |
| Individual Study for Final Examination | 1 | 30 | 30 |
| Laboratory Examination | 1 | 30 | 30 |
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| Contribution of Learning Outcomes to Programme Outcomes |
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| * Contribution Level : 1 Very low 2 Low 3 Medium 4 High 5 Very High |
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Iğdır University, Iğdır / TURKEY • Tel (pbx): +90 476
226 13 14 • e-mail: info@igdir.edu.tr
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