| Course Unit Code | Course Unit Title | Type of Course Unit | Year of Study | Semester | Number of ECTS Credits | | BMB-22-123 | GENE CLONİNG | Elective | 1 | 2 | 6 |
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| Level of Course Unit |
| Second Cycle |
| Objectives of the Course |
| The main goal of this course is to provide background knowledge on gene cloning to the MSc students. |
| Name of Lecturer(s) |
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| Learning Outcomes |
| 1 | What is a gene? | | 2 | Why gene cloning? | | 3 | Which techniques are employed on gene cloning? | | 4 | Why gene cloning is important? |
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| Mode of Delivery |
| Daytime Class |
| Prerequisites and co-requisities |
| The aim of this course is to introduce basic gene cloning techniques to the MSc students. |
| Recommended Optional Programme Components |
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| Course Contents |
| What is a gene, Why gene cloning, Which techniques are employed on gene cloning, Why gene cloning is important. |
| Weekly Detailed Course Contents |
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| 1 | What is a gene? | | | | 2 | Vectors for Gene Cloning: Plasmids and Bacteriophages | | | | 3 | Introduction of DNA into Living Cells | | | | 4 | Identification of recombinants | | | | 5 | Cloning Vectors for E. coli | | | | 6 | Cloning Vectors for Eukaryotes | | | | 7 | How to Obtain a Clone of a Specific Gene | | | | 8 | Identification methods based on detection of the translation product of the cloned gene | | | | 9 | The Polymerase Chain Reaction | | | | 10 | Studying Gene Location and Structure | | | | 11 | Studying Gene Expression and Function | | | | 12 | Studying Genomes | | | | 13 | Q & A with students | | | | 14 | General Discussion | | |
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| Recommended or Required Reading |
| Gen Klonlama ve DNA Analizi: Fevzi Bardakcı, Ali Fazıl Yenidünya, Nazan Yılmaz Ekim 2013 / 6. Baskı
Temel ve İleri Moleküler Biyoloji Yöntemleri Genomik ve Proteomik Analizler: Prof. Dr. Güler Temizkan , Prof. Dr. Nazlı Arda. Nobel Kitapevi ISBN: 9786053353621 |
| Planned Learning Activities and Teaching Methods |
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| Assessment Methods and Criteria | |
| Report Preparation | 1 | 100 | | SUM | 100 | |
| Report Preparation | 1 | 100 | | SUM | 100 | | Term (or Year) Learning Activities | 40 | | End Of Term (or Year) Learning Activities | 60 | | SUM | 100 |
| | Language of Instruction | | English | | Work Placement(s) | |
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| Workload Calculation |
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| Midterm Examination | 1 | 2 | 2 |
| Final Examination | 1 | 2 | 2 |
| Self Study | 14 | 8 | 112 |
| Individual Study for Mid term Examination | 1 | 20 | 20 |
| Individual Study for Final Examination | 1 | 20 | 20 |
| Report | 1 | 10 | 10 |
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| Contribution of Learning Outcomes to Programme Outcomes |
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| * Contribution Level : 1 Very low 2 Low 3 Medium 4 High 5 Very High |
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Iğdır University, Iğdır / TURKEY • Tel (pbx): +90 476
226 13 14 • e-mail: info@igdir.edu.tr
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