Iğdır University Lifelong Learning Programme

Description of Individual Course Units

Course Unit Code	Course Unit Title	Type of Course Unit	Year of Study	Semester	Number of ECTS Credits
192000203102	MOLECULAR BIOLOGICAL METHODS	Compulsory	2	3	2

Level of Course Unit

Short Cycle

Objectives of the Course

In this course; It is aimed to provide students with the knowledge and skills to apply these techniques by providing information about basic molecular biological methods and application principles that enable structural and functional analysis of molecular structures in the cell.

Name of Lecturer(s)

Öğr.Gör.Ercan OĞUZ

Learning Outcomes

1	Explains the concepts of gene, genome, chromosomes, DNA, RNA and protein synthesis at molecular level.
2	Explains nucleic acid and protein isolation methods and applies them in laboratory studies.
3	Explains the polymerase chain reaction (PCR) and other PCR methods and applies them in laboratory studies.
4	Applies electrophoresis and modified methods in laboratory studies.
5	Explain the basic elements of Western Blotting and ELISA methods and apply them in laboratory studies.
6	Explains the concepts of gene expression and control, sequence analysis.
7	Explains cell culture and flow cytometry methods.

Mode of Delivery

Daytime Class

Prerequisites and co-requisities

Non

Recommended Optional Programme Components

Non

Course Contents

In this course, the main elements of molecular biological methods, working principles of devices, nucleic acid isolation, polymerase chain reactions, electrophoresis, blotting and hybridization methods, protein isolation, SDS-PAGE, western blotting, transformation, transfection, cutting with restriction enzymes, ligation reactions, ELISA , cell culture and flow cytometry topics are shown in practice and the applications of these methods are made by the students.

Weekly Detailed Course Contents

Week	Theoretical	Practice	Laboratory
1	*Molecular Biological Methods and Its Main Elements
2	*Nukleik asit izolasyonu
3	*Polimeraz Zincir Reaksiyonu, Modifikasyonları ve Elektroforez
4	*Sequence analysis and next generation sequencing techniques
5	*Molecular Cloning (Restriction enzymes, ligation)
6	*Transformasyon, transfeksiyon
7	*Midterm
8	*Blotting and Hybridization Methods
9	*Protein Isolation and SDS-PAGE
10	*Basics of Enzymatic Analysis
11	*Microarray, Microchip, Nanochip and Biochip
12	*Cell Culture Techniques
13	*Gene editing techniques
14	final exam

Recommended or Required Reading

Basic Methods In Molecular Biology, Davis, Leonard, Elsevier, 2012. Gen Klonlama ve DNA Analizi: Giriş, 5. Basımdan Çeviri, Çeviri Kurulu: Fevzi Bardakçı, Ali Fazıl Yenidünya, Nazan Yılmaz, 2009. Molecular Cloning: A Laboratory Manual, 4th Edition, Micheal R. Green, Joseph Sambrook, Cold Spring Harbor Laboratory Press, 2012.

Planned Learning Activities and Teaching Methods

Assessment Methods and Criteria

Term (or Year) Learning Activities	Quantity	Weight
Midterm Examination	1	100
SUM		100
End Of Term (or Year) Learning Activities	Quantity	Weight
Final Examination	1	100
SUM		100
Term (or Year) Learning Activities		40
End Of Term (or Year) Learning Activities		60
SUM		100

Language of Instruction

Work Placement(s)

Workload Calculation

Activities	Number	Time (hours)	Total Work Load (hours)
Midterm Examination	1	1	1
Final Examination	1	2	2
Attending Lectures	14	2	28
Question-Answer	14	2	28
Homework	6	2	12
TOTAL WORKLOAD (hours)			71

Contribution of Learning Outcomes to Programme Outcomes

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* Contribution Level : 1 Very low 2 Low 3 Medium 4 High 5 Very High