Iğdır University Lifelong Learning Programme

Description of Individual Course Units

Dersin Kodu	Dersin Adı	Dersin Türü	Yıl	Yarıyıl	AKTS
192000203102	MOLECULAR BIOLOGICAL METHODS	Zorunlu	2	3	2

Dersin Seviyesi

Ön Lisans

Dersin Amacı

In this course; It is aimed to provide students with the knowledge and skills to apply these techniques by providing information about basic molecular biological methods and application principles that enable structural and functional analysis of molecular structures in the cell.

Dersi Veren Öğretim Görevlisi/Görevlileri

Öğr.Gör.Ercan OĞUZ

Öğrenme Çıktıları

1	Explains the concepts of gene, genome, chromosomes, DNA, RNA and protein synthesis at molecular level.
2	Explains nucleic acid and protein isolation methods and applies them in laboratory studies.
3	Explains the polymerase chain reaction (PCR) and other PCR methods and applies them in laboratory studies.
4	Applies electrophoresis and modified methods in laboratory studies.
5	Explain the basic elements of Western Blotting and ELISA methods and apply them in laboratory studies.
6	Explains the concepts of gene expression and control, sequence analysis.
7	Explains cell culture and flow cytometry methods.

Öğrenim Türü

Daytime Class

Dersin Ön Koşulu Olan Dersler

Non

Ders İçin Önerilen Diğer Hususlar

Non

Dersin İçeriği

In this course, the main elements of molecular biological methods, working principles of devices, nucleic acid isolation, polymerase chain reactions, electrophoresis, blotting and hybridization methods, protein isolation, SDS-PAGE, western blotting, transformation, transfection, cutting with restriction enzymes, ligation reactions, ELISA , cell culture and flow cytometry topics are shown in practice and the applications of these methods are made by the students.

Haftalık Ayrıntılı Ders İçeriği

Hafta	Teorik	Uygulama	Laboratuvar
1	*Molecular Biological Methods and Its Main Elements
2	*Nukleik asit izolasyonu
3	*Polimeraz Zincir Reaksiyonu, Modifikasyonları ve Elektroforez
4	*Sequence analysis and next generation sequencing techniques
5	*Molecular Cloning (Restriction enzymes, ligation)
6	*Transformasyon, transfeksiyon
7	*Midterm
8	*Blotting and Hybridization Methods
9	*Protein Isolation and SDS-PAGE
10	*Basics of Enzymatic Analysis
11	*Microarray, Microchip, Nanochip and Biochip
12	*Cell Culture Techniques
13	*Gene editing techniques
14	final exam

Ders Kitabı / Malzemesi / Önerilen Kaynaklar

Basic Methods In Molecular Biology, Davis, Leonard, Elsevier, 2012. Gen Klonlama ve DNA Analizi: Giriş, 5. Basımdan Çeviri, Çeviri Kurulu: Fevzi Bardakçı, Ali Fazıl Yenidünya, Nazan Yılmaz, 2009. Molecular Cloning: A Laboratory Manual, 4th Edition, Micheal R. Green, Joseph Sambrook, Cold Spring Harbor Laboratory Press, 2012.

Planlanan Öğrenme Aktiviteleri ve Metodları

Değerlendirme

Yarıyıl (Yıl) İçi Etkinlikleri	Adet	Değer
Midterm Examination	1	100
TOPLAM		100
Yarıyıl(Yıl) Sonu Etkinlikler	Adet	Değer
Final Examination	1	100
TOPLAM		100
Term (or Year) Learning Activities		40
End Of Term (or Year) Learning Activities		60
TOPLAM		100

Dersin Sunulduğu Dil

Staj Durumu

İş Yükü Hesaplaması

Etkinlikler	Sayısı	Süresi (saat)	Toplam İş Yükü (saat)
Midterm Examination	1	1	1
Final Examination	1	2	2
Attending Lectures	14	2	28
Question-Answer	14	2	28
Homework	6	2	12
TOPLAM İŞ YÜKÜ (saat)			71

Program ve Öğrenme Çıktıları İlişkisi

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2

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3

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4

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5

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6

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7

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8

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9

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10

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11

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12

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13

PÇ
14

PÇ
15

ÖÇ1

ÖÇ2

ÖÇ3

ÖÇ4

ÖÇ5

ÖÇ6

ÖÇ7

* Katkı Düzeyi : 1 Çok düşük 2 Düşük 3 Orta 4 Yüksek 5 Çok yüksek