Course Unit Code | Course Unit Title | Type of Course Unit | Year of Study | Semester | Number of ECTS Credits | TBİ-23-164 | BasIc TechnIques In Molecular BIology | Elective | 1 | 2 | 6 |
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Level of Course Unit |
Third Cycle |
Objectives of the Course |
The aim of this course is to teach basic techniques currently used in molecular biology to the MSc students. |
Name of Lecturer(s) |
Dr. Öğr. Üyesi Kaan Hürkan |
Learning Outcomes |
1 | 1) Biological properties of the cells at a molecular level. | 2 | 2) Which experimental steps are involved in protein and nucleic acid studies. | 3 | 3) Comparison of different experimental approaches in molecular biology. | 4 | 4) Basic techniques in molecular biology. |
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Mode of Delivery |
Daytime Class |
Prerequisites and co-requisities |
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Recommended Optional Programme Components |
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Course Contents |
Biological properties of the cells at a molecular level, Which experimental steps are involved in protein and nucleic acid studies, Comparison of different experimental approaches in molecular biology, Basic techniques in molecular biology. |
Weekly Detailed Course Contents |
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1 | Introduction to Basic Equipment Used in Molecular Biology Laboratories | | | 2 | General Overview of the Techniques Used in Molecular Biology 1st Section: Physical Techniques | | | 3 | General Overview of the Techniques Used in Molecular Biology 2nd Section: Chemical Techniques | | | 4 | General Overview of the Techniques Used in Molecular Biology 3rd Section: Spectral Techniques | | | 5 | General Overview of the Techniques Used in Molecular Biology 4th Section: Techniques Used in Analyses | | | 6 | Isolation of Nucleic Acids 1st Section: DNA Isolation from Living Organisms | | | 7 | Isolation of Nucleic Acids 2nd Section: DNA Isolation from Fossils, Criminal Specimens, and Extracellular DNA Isolation | | | 8 | Isolation of Nucleic Acids 3rd Section: Total RNA and mRNA Isolation | | | 9 | Analysis of Nucleic Acids | | | 10 | Isolation of Proteins 1st Section: Basic Equipment, Biologic Materials, Storage Conditions | | | 11 | Isolation of Proteins 2nd Section: Isolation of Water-Soluble Proteins | | | 12 | Isolation of Proteins 3rd Section: Protein Isolation from Plant Tissues | | | 13 | Quantitative Analysis of Proteins | | | 14 | Introduction to Bioinformatics | | |
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Recommended or Required Reading |
Temizkan, G., Arda, N., (Ed.)., 2018. Temel ve İleri Moleküler Biyoloji Yöntemleri Genomik ve Proteomik Analizler. İstanbul: Nobel Tıp Kitabevleri. ISBN: 9786053353621
Temizkan G., Arda, N., (Ed.)., 2008. Moleküler Biyolojide Kullanılan Yöntemler. İstanbul: Nobel Tıp Kitabevleri. ISBN: 9789754205831
Walker, J. M., Rapley, R., (Ed.)., 2008. Molecular Biomethods Handbook. 2nd Ed. Totowa: Humana Press. ISBN: 9781603273749 |
Planned Learning Activities and Teaching Methods |
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Assessment Methods and Criteria | |
Midterm Examination | 1 | 100 | SUM | 100 | |
Report | 1 | 100 | SUM | 100 | Term (or Year) Learning Activities | 40 | End Of Term (or Year) Learning Activities | 60 | SUM | 100 |
| Language of Instruction | Turkish | Work Placement(s) | |
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Workload Calculation |
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Midterm Examination | 1 | 1 | 1 |
Final Examination | 1 | 2 | 2 |
Self Study | 14 | 8 | 112 |
Individual Study for Mid term Examination | 1 | 20 | 20 |
Individual Study for Final Examination | 1 | 20 | 20 |
Homework | 1 | 10 | 10 |
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Contribution of Learning Outcomes to Programme Outcomes |
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* Contribution Level : 1 Very low 2 Low 3 Medium 4 High 5 Very High |
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Iğdır University, Iğdır / TURKEY • Tel (pbx): +90 476
226 13 14 • e-mail: info@igdir.edu.tr
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