Description of Individual Course Units
Course Unit CodeCourse Unit TitleType of Course UnitYear of StudySemesterNumber of ECTS Credits
TBİ-23-164BasIc TechnIques In Molecular BIology Elective126
Level of Course Unit
Third Cycle
Objectives of the Course
The aim of this course is to teach basic techniques currently used in molecular biology to the MSc students.
Name of Lecturer(s)
Dr. Öğr. Üyesi Kaan Hürkan
Learning Outcomes
11) Biological properties of the cells at a molecular level.
22) Which experimental steps are involved in protein and nucleic acid studies.
33) Comparison of different experimental approaches in molecular biology.
44) Basic techniques in molecular biology.
Mode of Delivery
Daytime Class
Prerequisites and co-requisities
Recommended Optional Programme Components
Course Contents
Biological properties of the cells at a molecular level, Which experimental steps are involved in protein and nucleic acid studies, Comparison of different experimental approaches in molecular biology, Basic techniques in molecular biology.
Weekly Detailed Course Contents
WeekTheoreticalPracticeLaboratory
1Introduction to Basic Equipment Used in Molecular Biology Laboratories
2General Overview of the Techniques Used in Molecular Biology 1st Section: Physical Techniques
3General Overview of the Techniques Used in Molecular Biology 2nd Section: Chemical Techniques
4General Overview of the Techniques Used in Molecular Biology 3rd Section: Spectral Techniques
5General Overview of the Techniques Used in Molecular Biology 4th Section: Techniques Used in Analyses
6Isolation of Nucleic Acids 1st Section: DNA Isolation from Living Organisms
7Isolation of Nucleic Acids 2nd Section: DNA Isolation from Fossils, Criminal Specimens, and Extracellular DNA Isolation
8Isolation of Nucleic Acids 3rd Section: Total RNA and mRNA Isolation
9Analysis of Nucleic Acids
10Isolation of Proteins 1st Section: Basic Equipment, Biologic Materials, Storage Conditions
11Isolation of Proteins 2nd Section: Isolation of Water-Soluble Proteins
12Isolation of Proteins 3rd Section: Protein Isolation from Plant Tissues
13Quantitative Analysis of Proteins
14Introduction to Bioinformatics
Recommended or Required Reading
Temizkan, G., Arda, N., (Ed.)., 2018. Temel ve İleri Moleküler Biyoloji Yöntemleri Genomik ve Proteomik Analizler. İstanbul: Nobel Tıp Kitabevleri. ISBN: 9786053353621 Temizkan G., Arda, N., (Ed.)., 2008. Moleküler Biyolojide Kullanılan Yöntemler. İstanbul: Nobel Tıp Kitabevleri. ISBN: 9789754205831 Walker, J. M., Rapley, R., (Ed.)., 2008. Molecular Biomethods Handbook. 2nd Ed. Totowa: Humana Press. ISBN: 9781603273749
Planned Learning Activities and Teaching Methods
Assessment Methods and Criteria
Term (or Year) Learning ActivitiesQuantityWeight
Midterm Examination1100
SUM100
End Of Term (or Year) Learning ActivitiesQuantityWeight
Report1100
SUM100
Term (or Year) Learning Activities40
End Of Term (or Year) Learning Activities60
SUM100
Language of Instruction
Turkish
Work Placement(s)
Workload Calculation
ActivitiesNumberTime (hours)Total Work Load (hours)
Midterm Examination111
Final Examination122
Self Study148112
Individual Study for Mid term Examination12020
Individual Study for Final Examination12020
Homework11010
TOTAL WORKLOAD (hours)165
Contribution of Learning Outcomes to Programme Outcomes
PO
1
PO
2
PO
3
PO
4
PO
5
PO
6
LO1      
LO2      
LO3      
LO4      
* Contribution Level : 1 Very low 2 Low 3 Medium 4 High 5 Very High
 
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