Iğdır University Lifelong Learning Programme

Description of Individual Course Units

Course Unit Code	Course Unit Title	Type of Course Unit	Year of Study	Semester	Number of ECTS Credits
BKO-23-100	LABORATORY METHODS IN PHYTOBACTERIOLOGY	Elective	1	2	6

Level of Course Unit

Second Cycle

Objectives of the Course

The general objective of this course is to learn about isolation of the bactera from diseased plant tissues by using general or selective medium, obtaining of pure bacterial culture and storing techniguee, hiper sensitivity and pathogenisity tests and some of the calassical identification techniques.

Name of Lecturer(s)

Yrd. Doç. Dr. Mesude Figen DÖNMEZ

Learning Outcomes

1	To understand the basic theory and concepts of phytobacteriology.
2	To learn knowledge about isolation of the bactera from diseased plant tissues.
3	To learn knowledge about obtaining of pure bacterial culture and storing techniguee.
4	To learn knowledge about hiper sensitivity and pathogenisity tests.
5	To learn knowledge about calassical identification techniques.

Mode of Delivery

Daytime Class

Prerequisites and co-requisities

None

Recommended Optional Programme Components

None

Course Contents

To learn about isolation of the bactera from diseased plant tissues by using general or selective medium, obtaining of pure bacterial culture and storing techniguee, hiper sensitivity and pathogenisity tests and some of the calassical identification techniques.

Weekly Detailed Course Contents

Week	Theoretical	Practice	Laboratory
1	General information on cell and colony morphology of plaant pathogenic bacteria
2	Reproduction, chemical and physiological structure and nutrition of bacteria
3	Classification of Prokaryotes
4	Introduction of the simptoms of plant bacterial diseases
5	Inoculation, reproduction, and transmission of plant pathogenic bacteria and simptom evolution on plants
6	Introduction of general aspects on the basis of Genus of plant pathogenic bacteria
7	Midterm Exam
8	Introduction of basic equipmants of bacteriology laboratories, preparation of standart, semiselective and selective media.
9	Sampling from diseased plants, learning the isolation and purification techniques from soil, water, seeds and plant organs
10	Long –term preservation techniques of bacterial cultures.
11	Basic identification methods for distinguish the different Genera of Prokaryotes (Bacterial cell and colony morphology)
12	Basic identification methods for distinguish the different Genera of Prokaryotes (Physiological and biochemical tests)
13	Inoculum production, counting bacteria and pathogenicity tests
14	Final Exam
15
16

Recommended or Required Reading

Plant Pathology, Fouth Edition. Academic Pres.San Diego, California. USA. Agrios, G. N., 1997.•Fitobakteriyoloji. Meta Basım Matbaacılık Hizmetleri. Bornova, İzmir. Saygılı, H., Şahin, F. ve Aysan, Y., 2006. Laboratory Guide for Identification of Plant Pathogenic Bacteria. APS Pres. The American Phytopathological Society. Schaad, N. V., 1994.•Laboratory Guide for Identification of Plant Pathogenic Bacteria. APS Pres. The American Phytopathological Society. Schaad, N. V., Jones, J. B. and Chun, W., 2001.•Methods for General and Molecular Bacteriology. American Society for Microbiology, Washington, D. C. Gerhardt, P., Murray, R. G. E., Wood, W. A. and Krieg, N. R., 2004.•Molecular Cloning 1, A Laboratory Manual, Second Edition, Cold Spring Harbor Laboratory Pres. Sambrook, J., Fritsch, E. F. and Maniatis, T., 1989.•Molecular Cloning 2, A Laboratory Manual, Second Edition, Cold Spring Harbor Laboratory Pres. Sambrook, J., Fritsch, E. F. and Maniatis, T., 1989.•Molecular Cloning 3, A Laboratory Manual, Second Edition, Cold Spring Harbor Laboratory Pres. Sambrook, J., Fritsch, E. F. and Maniatis, T., 1989.

Planned Learning Activities and Teaching Methods

Assessment Methods and Criteria

Term (or Year) Learning Activities	Quantity	Weight
SUM		0
End Of Term (or Year) Learning Activities	Quantity	Weight
SUM		0
SUM		0

Language of Instruction

Work Placement(s)

None

Workload Calculation

Activities	Number	Time (hours)	Total Work Load (hours)
Midterm Examination	1	1	1
Final Examination	1	2	2
Self Study	14	10	140
Individual Study for Mid term Examination	1	15	15
Individual Study for Final Examination	1	20	20
Homework	1	10	10
TOTAL WORKLOAD (hours)			188

Contribution of Learning Outcomes to Programme Outcomes

	PO 1	PO 2	PO 3	PO 4	PO 5	PO 6	PO 7	PO 8	PO 9	PO 10
LO1
LO2
LO3
LO4
LO5

* Contribution Level : 1 Very low 2 Low 3 Medium 4 High 5 Very High