Description of Individual Course Units
Course Unit CodeCourse Unit TitleType of Course UnitYear of StudySemesterNumber of ECTS Credits
TAB-23-112INSTRUMENTAL ANALYSIS TECHNIQUES USED FOR DETERMINATION OF PLANT COMPONENTSElective126
Level of Course Unit
Second Cycle
Objectives of the Course
Intrumental analysis techniques have been used for determination of plant constituents, recently. These tecniques are fundamental in biology, agriculture and food technology. In this lecture, student will learn theoritical basis of intrumental tools and will be able to use these equipments. Plant aromatic contituents, pesticide residues and other components will easily be determined at the result of the class.
Name of Lecturer(s)
Doç. Dr. Ahmet Metin Kumlay
Learning Outcomes
1Interpretation of results
2Theoretical knowledge about plant components and biochemistry
3Theoretical and practical information about refractometry, polarimetry spectrophotometry (UV/VIS)
4Detailed information about chromatographic techniques and their usage on plants
5Theoretical and practical information about HPLC, GC, MS, GC-MS, LC-MS and their usage on plants
6Application of Electrophoretic Techniques on Plants
Mode of Delivery
Daytime Class
Prerequisites and co-requisities
None
Recommended Optional Programme Components
None
Course Contents
Basic principals and theory of instrumental techniques, theory and application of Refractometry and Polarimetry, theory and application of Spectrophotometry (UV/VIS), theory and application of Chromatography, theory and application of Electrophoretic techniques.
Weekly Detailed Course Contents
WeekTheoreticalPracticeLaboratory
1Basic Principals of Instrumental Analysis Techniques
2Refractometry and Polarimetry
3Spectrophotometry (UV/VIS)
4General Principles of High Performance Liquid Chromatography (HPLC)
5Detailed Information about HPLC instrument
6HPLC Applications on Plants (Carbohydrates, Lipids, Vitamines)
7HPLC Applications on Plants (Mycotoxines, Pesticide Residues, Amino Acids)
8Mid-Term Exam
9Basic Principles af Gas Chromatography (GC) and Mass Spectrophotometry (MS)
10GC and MS applications on plants
11GC-MS and LC-MS applications on plants
12GC-MS and LC-MS applications on plants
13Basic Principals of Electrophoretic Techniques
14Application of Electrophoretic Techniques on Plants
15Application of Protein and DNA Electrophoresis Techniques on Plants
16Final Exam
Recommended or Required Reading
1.Enstrümental Gıda Analizleri - I – Yüksek Basınç Sıvı Kromatografisi. Prof. Dr. Yaşar Hışıl. Ege Üniversitesi Mühendislik Fakültesi Ders Kitapları Yayın No:31, İzmir, 2004. 2.Enstrümental Gıda Analizleri - II – Gaz, İnce-Tabaka, Kağıt Kromatografileri ve Elektroforez. Prof. Dr. Yaşar Hışıl. Ege Üniversitesi Mühendislik Fakültesi Ders Kitapları Yayın No:30, İzmir, 2004. 3.Enstrümental Gıda Analizleri. Prof. Dr. Hasan Yetim, Atatürk Üniversitesi Ziraat fakültesi Ders Yayınları No: 224, Erzurum, 2002
Planned Learning Activities and Teaching Methods
Assessment Methods and Criteria
Term (or Year) Learning ActivitiesQuantityWeight
Midterm Examination1100
SUM100
End Of Term (or Year) Learning ActivitiesQuantityWeight
Final Examination1100
SUM100
Term (or Year) Learning Activities50
End Of Term (or Year) Learning Activities50
SUM100
Language of Instruction
Turkish
Work Placement(s)
None
Workload Calculation
ActivitiesNumberTime (hours)Total Work Load (hours)
Midterm Examination155
Final Examination155
Attending Lectures14228
Laboratory14228
Writing Paper13030
Individual Study for Mid term Examination13030
Individual Study for Final Examination13030
Laboratory Examination13030
TOTAL WORKLOAD (hours)186
Contribution of Learning Outcomes to Programme Outcomes
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1
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9
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10
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11
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12
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13
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14
PO
15
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* Contribution Level : 1 Very low 2 Low 3 Medium 4 High 5 Very High
 
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