Description of Individual Course Units
Course Unit CodeCourse Unit TitleType of Course UnitYear of StudySemesterNumber of ECTS Credits
TAB-23-107PLANT BIOTECHNOLOGY AND GENETIC ENGINEERING APPLICATIONSElective126
Level of Course Unit
Second Cycle
Objectives of the Course
The course offers knowledge in plant biotechnological methodology as well as conventional plant breeding. Methods such as plants as production systems and transgene technology, are used. Emphasis is on biotechnology and molecular breeding of crop plant species but examples are also taken from the other agricultural products. In addition to the potential of plant biotechnology, the course highlights ethical and legal aspects as well as ecological considerations associated with these technologies.
Name of Lecturer(s)
Doç. Dr. Ahmet Metin KUMLAY
Learning Outcomes
1genetic markers and advantages and disadvantages of the transgenic plants.
2the molecular structure of chromosomes, genes and DNA and protein synthesis.
3the main steps in plant genetic engineering and isolation methods of DNA and RNA, the direct or indirect gene transfer methods to plants.
4plant genetic engineering applications; question the reasons of plant genetic engineering applications, herbicide, pest or disease tolerant engineered plants, stress resistant plants etc.
5plant genetic engineering applications; question the reasons of plant genetic engineering applications, herbicide, pest or disease tolerant engineered plants, stress resistant plants etc.
6plant genetic engineering applications; question the reasons of plant genetic engineering applications, herbicide, pest or disease tolerant engineered plants, stress resistant plants etc.
Mode of Delivery
Daytime Class
Prerequisites and co-requisities
None
Recommended Optional Programme Components
None
Course Contents
The molecular structure of chromosomes, genes and DNA and protein synthesis, the main steps in plant genetic engineering, DNA and RNA isolation theoretically; clone the gene of interest theoretically and manipulate the gene to be expressed in a plant, compare the plant tissue culture types in terms of suitability for gene transfer, the direct or indirect gene transfer methods to plants; Agrobacterium-mediated gene transfer, explain the events during T-DNA transfer and its integration to the plant genome, explain and compare the direct gene transfer methods to plants, plant genetic engineering applications; question the reasons of plant genetic engineering applications, herbicide, pest or disease tolerant engineered plants, stress resistant plants etc., genetic markers and analyses, the advantages and disadvantages of the transgenic plants, debate on concerns about the production of transgenic plants and their products.
Weekly Detailed Course Contents
WeekTheoreticalPracticeLaboratory
1Molecular structures of DNA and genes
2Tools of Recombinant DNA Technology: Restriction endonucleases, Ligase, Cloning Vectors
3Plant DNA izolation, Principles of genomic DNA cloning
4Plant DNA isolation, cDNA synthesis, cDNA cloning techniques
5Techniques for plant transformation Agrobacterium-mediated gene transfer
6Techniques for plant transformation- Direct gene transfer methods
7Plant transformation and binary vectors
8Mid-Term Exam
9Development of herbisite resistance plants and pest resistant plants
10Reducing the effects of viral disease and other diseases in plants-Production of male-sterile plants
11Genetic manipulation of stress tolerance in plants and some application examples
12The improvement of crop yield and quality
13Antisense RNA technology
14Molecular markers and genetic analysis methods
15Molecular farming, Ethical and legal aspects of biotechnology
16Final Exam
Recommended or Required Reading
Bitki Biyoteknolojisi-Genetik Mühendisliği ve Uygulamaları. Editörler: Sebahattin Özcan, Ekrem Gürel ve Mehmet Babaoğlu. Selçuk Üniversitesi Vakfı Yayınları, Konya, 2001. Gen Klonlama ve DNA Analizi. T. A. Brown. (Çeviri Kurulu: Fevzi Bardakcı, Ali Fazıl Yenidünya, Nazan Yılmaz). Nobel Yayınları, Ankara, 2009.
Planned Learning Activities and Teaching Methods
Assessment Methods and Criteria
Term (or Year) Learning ActivitiesQuantityWeight
Midterm Examination1100
SUM100
End Of Term (or Year) Learning ActivitiesQuantityWeight
Final Examination1100
SUM100
Term (or Year) Learning Activities50
End Of Term (or Year) Learning Activities50
SUM100
Language of Instruction
Turkish
Work Placement(s)
None
Workload Calculation
ActivitiesNumberTime (hours)Total Work Load (hours)
Midterm Examination155
Final Examination155
Attending Lectures14228
Practice14228
Writing Paper13030
Criticising Paper12020
Individual Study for Mid term Examination13030
Individual Study for Final Examination14040
TOTAL WORKLOAD (hours)186
Contribution of Learning Outcomes to Programme Outcomes
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* Contribution Level : 1 Very low 2 Low 3 Medium 4 High 5 Very High
 
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