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Description of Individual Course UnitsCourse Unit Code | Course Unit Title | Type of Course Unit | Year of Study | Semester | Number of ECTS Credits | TAB-23-107 | PLANT BIOTECHNOLOGY AND GENETIC ENGINEERING APPLICATIONS | Elective | 1 | 2 | 6 |
| Level of Course Unit | Second Cycle | Objectives of the Course | The course offers knowledge in plant biotechnological methodology as well as conventional plant breeding. Methods such as plants as production systems and transgene technology, are used. Emphasis is on biotechnology and molecular breeding of crop plant species but examples are also taken from the other agricultural products. In addition to the potential of plant biotechnology, the course highlights ethical and legal aspects as well as ecological considerations associated with these technologies. | Name of Lecturer(s) | Doç. Dr. Ahmet Metin KUMLAY | Learning Outcomes | 1 | genetic markers and advantages and disadvantages of the transgenic plants. | 2 | the molecular structure of chromosomes, genes and DNA and protein synthesis. | 3 | the main steps in plant genetic engineering and isolation methods of DNA and RNA, the direct or indirect gene transfer methods to plants. | 4 | plant genetic engineering applications; question the reasons of plant genetic engineering applications, herbicide, pest or disease tolerant engineered plants, stress resistant plants etc. | 5 | plant genetic engineering applications; question the reasons of plant genetic engineering applications, herbicide, pest or disease tolerant engineered plants, stress resistant plants etc. | 6 | plant genetic engineering applications; question the reasons of plant genetic engineering applications, herbicide, pest or disease tolerant engineered plants, stress resistant plants etc. |
| Mode of Delivery | Daytime Class | Prerequisites and co-requisities | None | Recommended Optional Programme Components | None | Course Contents | The molecular structure of chromosomes, genes and DNA and protein synthesis, the main steps in plant genetic engineering, DNA and RNA isolation theoretically; clone the gene of interest theoretically and manipulate the gene to be expressed in a plant, compare the plant tissue culture types in terms of suitability for gene transfer, the direct or indirect gene transfer methods to plants; Agrobacterium-mediated gene transfer, explain the events during T-DNA transfer and its integration to the plant genome, explain and compare the direct gene transfer methods to plants, plant genetic engineering applications; question the reasons of plant genetic engineering applications, herbicide, pest or disease tolerant engineered plants, stress resistant plants etc., genetic markers and analyses, the advantages and disadvantages of the transgenic plants, debate on concerns about the production of transgenic plants and their products.
| Weekly Detailed Course Contents | |
1 | Molecular structures of DNA and genes | | | 2 | Tools of Recombinant DNA Technology: Restriction endonucleases, Ligase, Cloning Vectors | | | 3 | Plant DNA izolation, Principles of genomic DNA cloning | | | 4 | Plant DNA isolation, cDNA synthesis, cDNA cloning techniques | | | 5 | Techniques for plant transformation Agrobacterium-mediated gene transfer | | | 6 | Techniques for plant transformation- Direct gene transfer methods | | | 7 | Plant transformation and binary vectors | | | 8 | Mid-Term Exam | | | 9 | Development of herbisite resistance plants and pest resistant plants | | | 10 | Reducing the effects of viral disease and other diseases in plants-Production of male-sterile plants | | | 11 | Genetic manipulation of stress tolerance in plants and some application examples | | | 12 | The improvement of crop yield and quality | | | 13 | Antisense RNA technology | | | 14 | Molecular markers and genetic analysis methods | | | 15 | Molecular farming, Ethical and legal aspects of biotechnology | | | 16 | Final Exam | | |
| Recommended or Required Reading | Bitki Biyoteknolojisi-Genetik Mühendisliği ve Uygulamaları. Editörler: Sebahattin Özcan, Ekrem Gürel ve Mehmet Babaoğlu. Selçuk Üniversitesi Vakfı Yayınları, Konya, 2001.
Gen Klonlama ve DNA Analizi. T. A. Brown. (Çeviri Kurulu: Fevzi Bardakcı, Ali Fazıl Yenidünya, Nazan Yılmaz). Nobel Yayınları, Ankara, 2009.
| Planned Learning Activities and Teaching Methods | | Assessment Methods and Criteria | |
Midterm Examination | 1 | 100 | SUM | 100 | |
Final Examination | 1 | 100 | SUM | 100 | Term (or Year) Learning Activities | 50 | End Of Term (or Year) Learning Activities | 50 | SUM | 100 |
| Language of Instruction | Turkish | Work Placement(s) | None |
| Workload Calculation | |
Midterm Examination | 1 | 5 | 5 | Final Examination | 1 | 5 | 5 | Attending Lectures | 14 | 2 | 28 | Practice | 14 | 2 | 28 | Writing Paper | 1 | 30 | 30 | Criticising Paper | 1 | 20 | 20 | Individual Study for Mid term Examination | 1 | 30 | 30 | Individual Study for Final Examination | 1 | 40 | 40 | |
Contribution of Learning Outcomes to Programme Outcomes | LO1 | 5 | 5 | 4 | 5 | 4 | 4 | 5 | 5 | 4 | | | | | | | LO2 | 4 | 4 | 5 | 4 | 5 | 5 | 4 | 4 | 5 | | | | | | | LO3 | 5 | 5 | 4 | 5 | 4 | 4 | 5 | 4 | 4 | | | | | | | LO4 | 4 | 4 | 5 | 4 | 5 | 5 | 5 | 5 | 4 | | | | | | | LO5 | 5 | 5 | 4 | 5 | 4 | 5 | 4 | 4 | 5 | | | | | | | LO6 | 4 | 4 | 4 | 4 | 5 | 4 | 4 | 5 | 5 | | | | | | |
| * Contribution Level : 1 Very low 2 Low 3 Medium 4 High 5 Very High |
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Iğdır University, Iğdır / TURKEY • Tel (pbx): +90 476
226 13 14 • e-mail: info@igdir.edu.tr
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