| Course Unit Code | Course Unit Title | Type of Course Unit | Year of Study | Semester | Number of ECTS Credits | | TBT-23-107 | BİTKİLERDE MOLEKÜLER MARKÖRLER VE DNA PARMAK İZİ | Elective | 1 | 2 | 6 |
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| Level of Course Unit |
| Second Cycle |
| Objectives of the Course |
| The aim of this course is to introduce the molecular markers used for genotyping of plants and teach DNA fingerprinting application to the MSc students. |
| Name of Lecturer(s) |
| Dr. Öğr. Üyesi Kaan Hürkan |
| Learning Outcomes |
| 1 | 1) Polymorphism and its importance | | 2 | 2) Molecular markers | | 3 | 3) Restriction digest and Polymerase Chain Reaction | | 4 | 4) Gel electrophoresis techniques | | 5 | 5) DNA fingerprinting in plants |
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| Mode of Delivery |
| Daytime Class |
| Prerequisites and co-requisities |
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| Recommended Optional Programme Components |
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| Course Contents |
| Polymorphism and its importance, Molecular markers, Restriction digest and Polymerase Chain Reaction, Gel electrophoresis techniques, DNA fingerprinting in plants |
| Weekly Detailed Course Contents |
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| 1 | DNA: An Important Source of Variation in Eukaryotic Genomes | | | | 2 | Detecting DNA Variation by Molecular Markers | | | | 3 | Introduction of Laboratory Equipment | | | | 4 | Isolation, Purification and Quantitative Analysis of Plant DNA | | | | 5 | Basic Molecular Techniques 1st Section: Restriction of DNA, Polymerase Chain Reaction, DNA Sequencing | | | | 6 | Basic Molecular Techniques 2nd Section: Agarose Gel Electrophoresis, Detecting DNA on Gel, PCR With Microsatellites, AFLP | | | | 7 | Evaluating Molecular Marker Data | | | | 8 | DNA Fingerprinting Applications on Plant Science | | | | 9 | Linkage Analysis and Genetic Maps | | | | 10 | Which Marker for What Purpose? | | | | 11 | Next Generation Sequencing 1st Section: Fundamentals, Techniques | | | | 12 | Next Generation Sequencing 2nd Section: Library Preparation | | | | 13 | Computer Software Types for Analysis of Molecular Markers | | | | 14 | Websites for Analysis of Molecular Markers | | |
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| Recommended or Required Reading |
| Henry, J. R., (Ed.), 2012. Molecular Markers in Plants. New Jersey: Wiley-Blackwell. ISBN: 9780470959510
Weising, K. and Nybom, H. and Pfenninger, M. and Wolff, K. and Kahl, G., 2005. DNA Fingerprinting in Plants: Principles, Methods, and Applications, Second Edition. Florida: CRC Press. ISBN: 9781420040043
Henry, J. R., (Ed.), 2001. Plant Genotyping The DNA Fingerprinting of Plants. Oxfordshire: Cabi Publishing. ISBN: 9780851995151 |
| Planned Learning Activities and Teaching Methods |
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| Assessment Methods and Criteria | |
| Midterm Examination | 1 | 100 | | SUM | 100 | |
| Report | 1 | 100 | | SUM | 100 | | Term (or Year) Learning Activities | 40 | | End Of Term (or Year) Learning Activities | 60 | | SUM | 100 |
| | Language of Instruction | | Turkish | | Work Placement(s) | |
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| Workload Calculation |
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| Midterm Examination | 1 | 1 | 1 |
| Final Examination | 1 | 2 | 2 |
| Self Study | 14 | 8 | 112 |
| Individual Study for Mid term Examination | 1 | 20 | 20 |
| Individual Study for Final Examination | 1 | 20 | 20 |
| Homework | 1 | 10 | 10 |
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| Contribution of Learning Outcomes to Programme Outcomes |
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| * Contribution Level : 1 Very low 2 Low 3 Medium 4 High 5 Very High |
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Iğdır University, Iğdır / TURKEY • Tel (pbx): +90 476
226 13 14 • e-mail: info@igdir.edu.tr
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