| Course Unit Code | Course Unit Title | Type of Course Unit | Year of Study | Semester | Number of ECTS Credits | | BMB-23-150 | Advanced molecular bIology laboratory technIques | Elective | 1 | 1 | 6 |
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| Level of Course Unit |
| Second Cycle |
| Objectives of the Course |
| To teach laboratory techniques about Molecular Biology, Molecular Genetics, Genetic Engineering, Microbiology and Biotechnology |
| Name of Lecturer(s) |
| Dr. Öğr. Üyesi Uğur GÜLLER |
| Learning Outcomes |
| 1 | 1) Students will be able to obtain information about the methods used in molecular biology
2) Students will be able to learn the objectives and the points to be considered in the use of the methods used in molecular biology.
3) Students will be able to learn theoretical knowledge about preparation of solution and buffer related to the methods
4) Students will be able to learn how to evaluate the obtained data
5) Students will be able to propose solutions to the problems related to molecular biology and genetics. |
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| Mode of Delivery |
| Daytime Class |
| Prerequisites and co-requisities |
| none |
| Recommended Optional Programme Components |
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| Course Contents |
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| Weekly Detailed Course Contents |
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| 1 | Various databases related to molecular biology and genetics and their usage | | | | 2 | Basic information for primer and probe design | | | | 3 | DNA isolation and DNA analysis (electrophoresis and band reading / spectrophotometric measurements) | | | | 4 | PCR solutions and PCR procedures | | | | 5 | Restriction Fragment Length Polymorphism (RFLP), | | | | 6 | Randomly Amplified Polymorphic DNA (RAPD), Amplified Fragment Length Polymorphism (AFLP) | | | | 7 | VNTR analysis, Single Nucleotide Polymorphism (SNP) | | | | 8 | RNA isolation and footnotes | | | | 9 | cDNA production | | | | 10 | Real Time PCR | | | | 11 | Promoter analysis and Bioinformatics applications | | | | 12 | Western blot technique -SDS gel preparation-Transfer the SDS gel proteins to the membrane (Blotting) -Blocking step, blocking non-specific binding inhibition (Blocking) -Specific antibodies incubation -Screening and interpretation of the result | | | | 13 | In situ hybridization method, purpose and application of the method, application of the method, encountered problems and solution proposals, screening and interpretation of the result | | | | 14 | Microarray analysis and types | | |
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| Recommended or Required Reading |
| 1) Setlow, J.K., 2007. Genetic Engineering, Principles and Methods. Plenum Press, New York.
2) Temizkan, G., Arda, N., 2004. Moleküler Biyolojide Kullanılan Yöntemler. Nobel Tıp Kitapevi.
3) Balbás, P., Lorence, A., 2004. Recombinant Gene Expression. Humana Press Inc. 999 Riverview Drive, Suite 208,Totowa, New Jersey 07512
4) Moleküler Biyoloji Teknikleri Laboratuvar El Kitabı, (2014), Aktif Yayınevi, ISBN:978-605-9940-01-6, Türkçe (Ders Kitabı). |
| Planned Learning Activities and Teaching Methods |
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| Assessment Methods and Criteria | |
| Midterm Examination | 1 | 100 | | SUM | 100 | |
| Final Examination | 1 | 100 | | SUM | 100 | | Term (or Year) Learning Activities | 50 | | End Of Term (or Year) Learning Activities | 50 | | SUM | 100 |
| | Language of Instruction | | Turkish | | Work Placement(s) | |
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| Workload Calculation |
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| Midterm Examination | 1 | 1 | 1 |
| Final Examination | 1 | 2 | 2 |
| Attending Lectures | 14 | 3 | 42 |
| Laboratory | 1 | 6 | 6 |
| Problem Solving | 1 | 12 | 12 |
| Discussion | 1 | 12 | 12 |
| Brain Storming | 1 | 12 | 12 |
| Report Preparation | 1 | 12 | 12 |
| Report Presentation | 1 | 12 | 12 |
| Project Preparation | 1 | 12 | 12 |
| Project Presentation | 1 | 6 | 6 |
| Seminar | 1 | 6 | 6 |
| Criticising Paper | 1 | 12 | 12 |
| Self Study | 1 | 12 | 12 |
| Report | 1 | 12 | 12 |
| Homework | 1 | 12 | 12 |
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| Contribution of Learning Outcomes to Programme Outcomes |
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| * Contribution Level : 1 Very low 2 Low 3 Medium 4 High 5 Very High |
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Iğdır University, Iğdır / TURKEY • Tel (pbx): +90 476
226 13 14 • e-mail: info@igdir.edu.tr
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