Iğdır University Lifelong Learning Programme

Description of Individual Course Units

Course Unit Code	Course Unit Title	Type of Course Unit	Year of Study	Semester	Number of ECTS Credits
BMB-23-168	RecombInant DNA Methods and ApplIcatIon FIelds	Elective	1	2	6

Level of Course Unit

Second Cycle

Objectives of the Course

Recombinant DNA technology methods mark a new epoch in biology. Recombinant DNA technology includes extremely important techniques in the production of transgenic plants and animals, genetically modified foods and several medicines and vaccines.

Name of Lecturer(s)

Dr. Öğr. Üyesi Namık KILINÇ

Learning Outcomes

-Establishing an association between the methods and recombinant products used in such areas as food, textile, agriculture and medicine, and having the competence to envisage possible future events - Gaining the skills necessary to recognize the advantages and disadvantages of the production of transgenic plants and animals, and to prevent the losses likely to arise

Mode of Delivery

Daytime Class

Prerequisites and co-requisities

none

Recommended Optional Programme Components

Course Contents

Weekly Detailed Course Contents

Week	Theoretical	Practice	Laboratory
1	Restriction endonucleases, plasmid cloning vectors, formation of gene library	none	none
2	Hybridization of gene library, Screening by immunological assay and by protein activity	none	none
3	Cloning DNA sequencing that encode eukaryotic proteins; Vectors for cloning large pieces of DNA, bacteriophage, cosmid, bacterial vector system	none	none
4	Genetic transformation of procaryotes; Chemical synthesis, sequencing and amplification of DNA	none	none
5	Manipulation of gene expression in procaryotes (gene expression from strong and regulatable promotors)	none	none
6	Fusion proteins	none	none
7	Translation expression vectors	none	none
8	Protein folding, DNA integration into the host chromosome	none	none
9	Heterolog protein production in Eukaryotic cell: Saccharomyces cerevisiae	none	none
10	Heterolog protein production in Eukaryotic cell: Baculavirus insect cell system	none	none
11	Heterolog protein production in Eukaryotic cell: mammalian cell systems	none	none
12	In-vitro mutagenesis	none	none
13	Protein engineering	none	none
14	Production of therapeutic agents, Production of vaccines	none	none

Recommended or Required Reading

Glick, B.R., Pasternak, J.J., Molecular Biotechnology (Principles and Applications of Recombinant DNA), ASM Press, 2003

Planned Learning Activities and Teaching Methods

Assessment Methods and Criteria

Term (or Year) Learning Activities	Quantity	Weight
Midterm Examination	1	100
SUM		100
End Of Term (or Year) Learning Activities	Quantity	Weight
Final Examination	1	100
SUM		100
Term (or Year) Learning Activities		50
End Of Term (or Year) Learning Activities		50
SUM		100

Language of Instruction

Turkish

Work Placement(s)

Workload Calculation

Activities	Number	Time (hours)	Total Work Load (hours)
Attending Lectures	14	3	42
Discussion	14	2	28
Question-Answer	14	2	28
Project Preparation	7	2	14
Project Presentation	7	2	14
Project Design/Management	7	2	14
Writing Paper	7	2	14
Criticising Paper	7	2	14
TOTAL WORKLOAD (hours)			168

Contribution of Learning Outcomes to Programme Outcomes

	PO 1	PO 2	PO 3	PO 4	PO 5	PO 6	PO 7	PO 8	PO 9	PO 10	PO 11	PO 12	PO 13
LO1	4	4	3	4	5	3	4	3	4	4	4	3	4

* Contribution Level : 1 Very low 2 Low 3 Medium 4 High 5 Very High