Course Unit Code | Course Unit Title | Type of Course Unit | Year of Study | Semester | Number of ECTS Credits | 182000204102 | MOLECULAR BIOLOGICAL METHODS | Compulsory | 2 | 4 | 3 |
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Level of Course Unit |
Short Cycle |
Objectives of the Course |
To learn the basics of analysis of molecular biology methods, to apply these techniques in laboratory conditions, to evaluate the results of the application, to express and discuss in writing and to optimize the laboratory application. |
Name of Lecturer(s) |
Öğr. Gör. Ulaş ÇALIŞIR |
Learning Outcomes |
1 | The general techniques used in the laboratory | 2 | Molecular biology calculations, measurements, sterilization techniques | 3 | DNA and RNA structure, properties and insulation | 4 | Uses molecular methods |
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Mode of Delivery |
Daytime Class |
Prerequisites and co-requisities |
none |
Recommended Optional Programme Components |
none |
Course Contents |
Nucleic acids (DNA-RNA) and Nucleic acid replication methods, Molecular biology of bacteriology parasitology and use of virology, Blotting techniques and their use, Principles of molecular epidemiology. |
Weekly Detailed Course Contents |
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1 | Molecular methods used in the laboratory | 4 | | 2 | Molecular biology calculations, measurements, sterilization techniques | 4 | | 3 | Physical and chemical methods, separation, purification and analysis methods, filtration, dialysis, freeze-drying (lyophilization), precipitation, enzyme application, centrifugation | 4 | | 4 | Structure and properties of DNA and RNA | 4 | | 5 | DNA isolation and analysis | 4 | | 6 | RNA isolation and analysis | 4 | | 7 | protein Isolation | 4 | | 8 | immunological methods | 4 | | 9 | Electrophoresis methods | 4 | | 10 | Polymerase chain reactions | 4 | | 11 | Southern Hibridizasyon | 4 | | 12 | Nouthern Hibridizasyon | 4 | | 13 | Nucleic acid hybridization techniques | 4 | | 14 | Uses molecular methods | 4 | |
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Recommended or Required Reading |
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Planned Learning Activities and Teaching Methods |
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Assessment Methods and Criteria | |
Midterm Examination | 1 | 100 | SUM | 100 | |
Final Examination | 1 | 100 | SUM | 100 | Term (or Year) Learning Activities | 40 | End Of Term (or Year) Learning Activities | 60 | SUM | 100 |
| Language of Instruction | Turkish | Work Placement(s) | none |
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Workload Calculation |
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Midterm Examination | 1 | 1 | 1 |
Final Examination | 1 | 2 | 2 |
Makeup Examination | 1 | 2 | 2 |
Quiz | 2 | 1 | 2 |
Laboratory | 14 | 3 | 42 |
Experiment | 7 | 4 | 28 |
Individual Study for Mid term Examination | 1 | 10 | 10 |
Individual Study for Final Examination | 1 | 10 | 10 |
Laboratory Midterm Examination | 1 | 2 | 2 |
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Contribution of Learning Outcomes to Programme Outcomes |
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* Contribution Level : 1 Very low 2 Low 3 Medium 4 High 5 Very High |
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Iğdır University, Iğdır / TURKEY • Tel (pbx): +90 476
226 13 14 • e-mail: info@igdir.edu.tr
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